(A)The average amplitude of BK-induced Ca2+ release from DKO cells transfected with EGFP together with PS1 and PS2 rescue constructs is shown as mean ± SD (n = number of cells analyzed). When compared to DKO cells transfected with EGFP alone, the amplitude of BK-in-duced Ca2+ response is significantly (***p < 0.05) smaller in DKO cells transfected with EGFP + PS1, EGFP + PS1-DE9, EGFP + PS1-D257A, and EGFP + PS2.
(B)The average size of the IO-sensitive Ca2+ pool is shown for DKO cells transiently transfected with EGFP and PS1 rescue constructs and for stably transfected DKO cells as mean ± SD (n = number of cells analyzed). When compared to DKO cells transfected with EGFP alone, the size of the IO-releasable Ca2+ pool is significantly (***p < 0.05) smaller in DKO cells transfected with EGFP + PS1, EGFP + PS1-DE9, and EGFP + PS1-D257A. The size of the IO-releasable Ca2+ pool is also significantly (***p < 0.05) smaller in HPS1, PS1-D257A, PS1-DE9, and HPS2 cell lines.