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Titrations of 0.2 mM (0.11 mol%) U-15N-C99 with either unlabeled CD147-CTD (A) or KCNE1 (negative control, B) at high detergent concentration as monitored by 600 MHz 1H-15N TROSY NMR spectra at 45 C. The sample buffer in both cases contained 250 mM imidazole and 9% LMPG (188 mM), pH 7.4 .