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. Author manuscript; available in PMC: 2012 Dec 21.
Published in final edited form as: J Am Chem Soc. 2011 Nov 22;133(50):20052–20055. doi: 10.1021/ja208050t

Figure 5.

Figure 5

Determining the catalytic contribution from the oxyan-ion hole in KSI. A) The second-order solution reaction of a KSI substrate with acetate ion. B) The second-order reaction of wild type KSI with the same substrate. C) The second-order reaction of the KSI oxyanion hole ablation mutant. D) Comparison of the second-order rate constant with the second-order nonenzymatic acetate-catalyzed reaction rate constant to measure the overall rate increase for KSI relative to the solution reaction that uses the same general base functionality. Comparison of reaction from the KSI E•S complex relative to the acetate-catalyzed reaction is given in Figure S7.