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Dissociation constants determined by fluorescence for Scn binding to CMB isoforms (n = 3–8)a
| FeIIICMB (by n) | Kd (μM) | Est. Std. Dev. |
|---|---|---|
| 3 | > 9 | -- |
| 4 | 2.36 | ±9.0×10−3 |
| 5 | 1.10 | ±2.6×10−2 |
| 6 | 0.654 | ±2.2×10−2 |
| 7 | 0.128 | ±1.0×10−3 |
| 8 | 0.280 | n.d.b |
Uncertainties were determined from the standard deviation of three independent titrations.
Minimal sample size allowed for only one binding experiment.
