Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Nat Chem Biol. 2011 Nov 27;8(1):125–132. doi: 10.1038/nchembio.721

a, Solubilized inclusion bodies of indicated CTDs obtained after expression in E. coli. b, c, UV-Vis (b) and X-band EPR spectra (c) of purified soluble Pol2-, Pol3- and Rev3-CTDs in absence of chaotropic agents. The inset in (b) shows non-heme iron and acid-labile sulfide contents. Error bars, s.d. (n ≥ 3). Samples in (c) were reduced with 2 mM sodium dithionite (2 min), EPR conditions: 9.458 GHz, 10 K and 2 mW microwave power.

a, Solubilized inclusion bodies of indicated CTDs obtained after expression in E. coli. b, c, UV-Vis (b) and X-band EPR spectra (c) of purified soluble Pol2-, Pol3- and Rev3-CTDs in absence of chaotropic agents. The inset in (b) shows non-heme iron and acid-labile sulfide contents. Error bars, s.d. (n ≥ 3). Samples in (c) were reduced with 2 mM sodium dithionite (2 min), EPR conditions: 9.458 GHz, 10 K and 2 mW microwave power.