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. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Nat Chem Biol. 2011 Nov 27;8(1):125–132. doi: 10.1038/nchembio.721

a, Alkaline agarose gel electrophoresis of DNA replication products of assay with purified proteins as indicated. A phosphorimaging scan is shown. ΔPIP refers to truncated Pol32 (Supplementary Fig. 11). All complexes contain wild-type Pol31. The right-most lane is a control with WT Pol δ but without PCNA. Size markers are indicated on the left. b, Assay as in a was carried out for 10 min, and with the indicated PCNA concentrations. c, Model for the roles of the PIP and CysA motifs for PCNA interaction with Pol δ in solution (Off DNA), and in assembly with substrate (On DNA).

a, Alkaline agarose gel electrophoresis of DNA replication products of assay with purified proteins as indicated. A phosphorimaging scan is shown. ΔPIP refers to truncated Pol32 (Supplementary Fig. 11). All complexes contain wild-type Pol31. The right-most lane is a control with WT Pol δ but without PCNA. Size markers are indicated on the left. b, Assay as in a was carried out for 10 min, and with the indicated PCNA concentrations. c, Model for the roles of the PIP and CysA motifs for PCNA interaction with Pol δ in solution (Off DNA), and in assembly with substrate (On DNA).