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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Oct 13;32(1):50–64. doi: 10.1161/ATVBAHA.111.238329

Figure 6. CD40 mediates vascular SMC apoptosis but has no effect on SMC proliferation and migration in vivo and in vitro.

Figure 6

Figure 6

Figure 6

Figure 6

Figure 6

Representative images showing double-staining of PCNA (A) and TUNEL (B) with SMα-actin in carotid arteries of chimeric mice at 7d after ligation, as well as their quantitative analysis (n=5 per group). Insert shows appropriate isotype control combined with DAPI staining. Scale bars: 50 μm. ** P<0.01 versus WT to WT group. (C) Cell proliferation measured by cell counting in WT and CD40−/− SMCs exposed to 10% FBS at the indicted time points. (D) Cell migration toward 10% FBS or PDGF-BB (20 ng/ml) measured by transwell chambers assay in WT and CD40−/− SMCs. Representative images of filters with DAPI-stained cells are shown (upper panel). Scale bar: 50 μm. Quantification of migrated cells is shown (lower panel). (E) Representative photomicrographs of DAPI staining in WT and CD40−/− SMCs treated with apoptosis inducer C2 ceramide (100 μM) for 5 hours, as well as quantitative analysis of cell apoptosis index (%). Arrows indicate the condensed or fragmented nuclei of apoptotic cells. Scale bars: 50 μm. ** P<0.01 versus WT group.