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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Oct 27;32(1):92–102. doi: 10.1161/ATVBAHA.111.238618

Figure 4. Validation of in vivo imaging by real time-PCR analysis of SRY containing L2G BMMCs transplanted into female recipient hearts.

Figure 4

(A) Ex vivo correlation between PCR cycle number (probing for SRY) for known number of male cells transplanted into female hearts demonstrates an R2 value of 0.99. (B) Regression analysis of BLI signal generated from hearts imaged in vivo (n=8) at different times following BMMC transplantation followed by real time PCR analysis for SRY demonstrates a robust correlation between BLI signal and SRY copy number (r2=0.95). Right axis gives expected cell number for given BLI signal based on ex vivo analysis depicted in (A).