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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2677–2685. doi: 10.1002/jcp.23010

Figure 3. Enforced expression of Osterix increases the expression of osteoblast related extracellular matrix protein genes.

Figure 3

hMSC were transfected with either PRLP2 control or PRLP2-Osterix retroviral supernatant and treated with BMP6 for four days. Quantitative real-time PCR was used to demonstrate the effects of Osterix expression on other duration-dependent genes in the absence of BMP2 and with suboptimal 2.5 nM and 10 nM BMP6. (A) Osterix (B) BSP (C) osteomodulin (D): Western blot was performed to demonstrate that Osterix increased BSP protein level in the osteogenic medium with BMP6 for 4 days at 0, 2.5 and 10 nM. The qPCR data represent the mean values ±SD of triplicate experiments normalized to the housekeeping gene β-actin. Statistical differences (*) of p < .05 between the Osterix-overexpressed and control PRLP2 cell lines were determined using a t-test.