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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2677–2685. doi: 10.1002/jcp.23010

Figure 4. Osterix is required for BMP6 induced hMSC osteogenic differentiation.

Figure 4

hMSC were transfected with mir-Osterix retroviral supernatant treated hMSC in the osteogenic medium with BMP6 for 4 days at 0, 2.5 and 10 nM Real-time quantitative PCR analysis was performed to demonstrate that knockdown of (A) Osterix on osteoblast associated genes (B) OMD and (C) BSP relative to the vector controls. (D) Western blot was performed to demonstrate that knockdown of Osterix decreased BSP protein level. The qPCR data represent the mean values ±SD of triplicate experiments normalized to the housekeeping gene β-actin. Statistical differences (*) of p < .05 between the Osterix knockdown and control PRLP2 cell lines were determined using t-test.