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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2677–2685. doi: 10.1002/jcp.23010

Figure 5. Osterix regulates hMSC osteoblastogenesis and proliferation.

Figure 5

(A) Left panel: Osterix-overexpressing and knockdown hMSC were treated with BMP6 for 96 hours at 0, 2.5 and 10 nM, and stained with Alizarin red S to evaluate calcium phosphate mineral, compare to the control cells. Right panel: Alizarin red S stained wells visualized using bright-field microscopy. (B): Transduced hMSC from donor 1 and donor 2 were cultured in assay medium with 20% FBS (growth conditions). Cumulative cell number was counted at 6, 12 and 18 days. Statistical differences (*) of p< .05 between the Osterix overexpression or knockdown and control PRLP2 cell lines were determined using t-test.