FIG. 2.

uPAR in vivo gene delivery via electroporation. Long-term overexpression of mouse uPAR was achieved in wild-type C57BL/6 mice by in vivo gene delivery accompanied with electroporation. Electroporation was performed after plasmid harboring uPAR was injected intradermally in the flank of the mice. Two plasmid injections on days 0 and 7 followed by in vivo electroporation were performed. In vivo mouse uPAR expression is indirectly shown by the persistent proteinuria in wild-type C57BL/6 mice. Albuminuria levels (μg/dL) normalized by creatininuria levels (mg/dL) were tested in male mice. In brief, under anesthesia, mouse uPAR plasmid (40 μg in PBS) was injected intradermally and bilaterally at the hind leg of the mice, followed by in vivo electroporation with Derma Vax DNA delivery system.