FIG. 5.

Mounting antibody responses against cytosolic, transmembrane, and receptor target antigens. (A) Units of integrated intensity of FLISA on uPAR transfected 293T cells and Mayven transfected Cos-7 with sera of mice immunized. FLISA (in-cell Western) was performed and the units of integrated intensity at channel 8 of an Odyssey Infrared Imager were graphed. (B) Serum titers (1:100 dilution) are shown as integrated intensity of titers of sera from mice immunized with plasmid REEP1 obtained in a FLISA (in-cell Western) assay compared with cells transfected with an irrelevant plasmid (control). Averages and standard deviations for five (uPAR and REEP) or three (Mayven) immunized mice of each group are shown. No Pr, no primary antibodies added, only secondary antibodies added. c: Pooled sera from mice collected prior to immunization.