FIG. 7.

Mounting antibody responses against vGPCR, which is a membrane protein, via in vivo electroporation. Low antibody responses against vGPCR was achieved by a single DNA immunization followed by in vivo electroporation that was augmented upon a boost. The plasmid encoding for vGPCR (20 μg in PBS) was injected intradermally followed by in vivo electroporation on the injection site. Average integrated intensity values of the 1:100 sera dilution are shown. Titers were assessed as discussed in Figure 6. Integrated intensity values for five immunized mice of each group are shown as averages and standard deviations. EP, DNA immunization followed by in vivo electroporation.