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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jul 26;1818(8):1985–1992. doi: 10.1016/j.bbamem.2011.07.028

Fig. 2. Gap junction remodeling in heart occurs in response to ischemia.

Fig. 2

Mouse hearts were excised and immediately fixed in formalin (CON) or first incubated without perfusion in non-oxygenated PBS for 30 min (ISCHEMIA). Paraffin embedded sections were co-labeled with a rabbit antibody to Total Cx43 (Sigma-Aldrich, St. Louis, MO) and a mouse antibody, CT1, which recognizes Cx43 unphosphorylated on S364/365 (FHCRC, Seattle, WA). Overlay panel shows the Total Cx43 antibody labeled with Alexa 546-conjugated secondary antibody in red and CT1 labeled with Alexa 488-conjugated secondary antibody in green.