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The transition states which lead to the four possible ortho-stereoisomers are shown above the reaction chromatograms. Background reaction: 2 mM diene and 70 mM dieneophile in a PBS solution for 24 hours at 298 K. DA_20_10 reaction: 50 μM protein, 0.5 mM diene, and 10 mM dieneophile in a PBS solution for 48 hours at 298K (16).
