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. Author manuscript; available in PMC: 2011 Dec 18.
Published in final edited form as: Cardiovasc Res. 2008 Feb 23;78(3):458–465. doi: 10.1093/cvr/cvn052

Figure 2.

Figure 2

PKD1 is bound to the second LIM domain of ENH1. Flag-tagged ENH1 (ENH1-Fl) or LIM deletion mutants (d1LIM-FL, d2LIM-FL, and d3LIM-FL) schematically presented in (A) were co-expressed with an HA-tagged PKD1 in 293T cells by transient transfection. From the 293T cell extract, either ENH1-FL or PKD1-HA was immunoprecipitated using anti-Flag (IP:Flag) or anti-HA (IP:HA) monoclonal antibodies, respectively. Immunoprecipitated proteins were analysed by WB using an HRP conjugated anti-Flag (WB:Flag) or anti-HA monoclonal antibodies (WB:HA).