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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Curr Opin Genet Dev. 2011 Sep 13;21(5):538–548. doi: 10.1016/j.gde.2011.08.003
Linear microscopy Microscopy using on a linear contrast mechanism (the signal scales linearly with the laser illumination intensity).
Nonlinear microscopy Microscopy using on a nonlinear contrast mechanism (the signal scales nonlinearly with the laser illumination intensity), including 2PEF, SHG, THG, CARS, and SRS signals.
Multiphoton microscopy Synonym to nonlinear microscopy.
1p 1-photon (corresponding to a linear excitation)
2p 2-photon (corresponding to a nonlinear excitation)
Contrast mechanisms:
1PEF 1-Photon Excited Fluorescence
2PEF 2-Photon Excited Fluorescence
SHG Second Harmonic Generation
THG Third Harmonic Generation
CARS Coherent Anti-stokes Raman Scattering
SRS Stimulated Raman Scattering
Microscope implementation:
Point-scanning 2p-microscopy Microscopy based on 2PEF signal using raster scanning of single point (also called TPLSM, 2p-LSM, 2p-microscopy, 2PEF microscopy, two-photon microscopy in the literature).
Light-sheet illumination Microscopy using light-sheet illumination in orthogonal geometry (also called SPIM, DSLM, Plane illumination microscopy,… in the litterature)
Light-sheet 2p-microscopy Microscopy based on 2PEF signal using light-sheet illumination (also called 2p-SPIM in [16])
Light-sheet 1p-microscopy Microscopy based on 1PEF signal using light-sheet illumination (also called 1p-SPIM in [16], DSLM in [20])
Multifocal Multiphoton Microscopy Microscopy based on 2PEF signal using raster scanning of multiple points