Linear microscopy |
Microscopy using on a linear contrast mechanism (the signal scales linearly with the laser illumination intensity). |
Nonlinear microscopy |
Microscopy using on a nonlinear contrast mechanism (the signal scales nonlinearly with the laser illumination intensity), including 2PEF, SHG, THG, CARS, and SRS signals. |
Multiphoton microscopy |
Synonym to nonlinear microscopy. |
1p |
1-photon (corresponding to a linear excitation) |
2p |
2-photon (corresponding to a nonlinear excitation) |
Contrast mechanisms: |
1PEF |
1-Photon Excited Fluorescence |
2PEF |
2-Photon Excited Fluorescence |
SHG |
Second Harmonic Generation |
THG |
Third Harmonic Generation |
CARS |
Coherent Anti-stokes Raman Scattering |
SRS |
Stimulated Raman Scattering |
Microscope implementation: |
Point-scanning 2p-microscopy |
Microscopy based on 2PEF signal using raster scanning of single point (also called TPLSM, 2p-LSM, 2p-microscopy, 2PEF microscopy, two-photon microscopy in the literature). |
Light-sheet illumination |
Microscopy using light-sheet illumination in orthogonal geometry (also called SPIM, DSLM, Plane illumination microscopy,… in the litterature) |
Light-sheet 2p-microscopy |
Microscopy based on 2PEF signal using light-sheet illumination (also called 2p-SPIM in [16]) |
Light-sheet 1p-microscopy |
Microscopy based on 1PEF signal using light-sheet illumination (also called 1p-SPIM in [16], DSLM in [20]) |
Multifocal Multiphoton Microscopy |
Microscopy based on 2PEF signal using raster scanning of multiple points |