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. Author manuscript; available in PMC: 2011 Dec 18.
Published in final edited form as: Mol Ecol Resour. 2009 Jan 22;9(3):958–960. doi: 10.1111/j.1755-0998.2008.02512.x

Table 1.

Characterization of 17 microsatellite loci in Culex restuans

Locus Primer sequences (5′-3′) M A Allele
range (bp)		 HO HE P Motif GenBank
Accession no.
Crest1T1.2d F: ATTAGCTTCCCAGGCTCTCC
R: CGGAAAGCGGAATCGATAG(H)		 3 13 142–166 0.83 0.89 0.34 CA10 FJ373274
Crest1T1.17 F: ACCACACCGATCATTATCCTC
R: GAAACATGGAAACGGCAAAC(N)		 3 8 131–153 0.72 0.73 0.58 GTT6 FJ373273
Crest1T1.30 F: TCTTCGCGCAGAAACGTC(F)
R: ACTGCTGCTGGACAGTACCC		 3 7 118–130 0.68 0.77 0.38 CA8 FJ373275
Crest1T2.21 L F: GCTGATCTTGGCTTGTGGTC(F)
R: AACTAAACAATGTCACGAGATACAAGG		 1 2 181–189* 0.14 0.17 0.29 GAGT5 FJ373276
Crest1T2.23 F: CAAGCCTTGGTCATGTGAG(H)
R: CGCGCCATTTTGTCTTTAC		 2 9 101–117 0.69 0.79 0.72 CA7 FJ373277
Crest1T2.33 F: AGGGCACATGTTAAGCCTTG
R: TCGGAAATCGAATGATGTTG(F)		 2 6 88–100 0.81 0.75 0.34 GA7 FJ373278
Crest1T2.54 F: TCTCCTGTACGCTTGTGTGC(H)
R: CCTGGCTGATCTTTGGACTG		 1 4 103–112 0.4 0.43 0.48 CAA6 FJ373279
Crest1T2.83a F: CCCATTCAAAGTTAAAAGATTGC(F)
R: GCCAAGACTGATTGGAAACG		 1 9 118–164 0.6 0.79 < 0.01 CAA5 FJ373280
Crest1T3.7 F: CAATCATCGAGGAGCATCAG(H)
R: TTGCGATGATCCTGTCGTAG		 1 6 132–152 0.42 0.43 0.62 CAA4 FJ373281
Crest1T4.76 F: CGATATGGGAACGCGTTAGG(F)
R: GCAGTTTGAGAGGCTCAACG		 3 3 174–178 0.16 0.33 < 0.01 TG6 FJ373282
Crest1T4.82 F: GAAGTGTGAGCTAGGGCAAG(N)
R: CAATTCAATGCCAAATACCG		 1 10 156–168 0.67 0.63 0.49 GT7 FJ373283
Crest1T5.52 F: GCGAGATTTCGTTGACAAAC
R: ACGAAGAGCCGAGGTACAAC(N)		 2 7 99–128 0.46 0.44 0.74 CAA6 FJ373284
Crest1T5.87 F: TGTTTATCCTGGAGGAAATGAG
R: CCAAAGCCTTTCCTGTCATC(F)		 2 20 159–204 0.83 0.87 0.28 GT9 FJ373285
Crest2T2.25 F: GACACGGAAGCGAAATTAAAC(N)
R: ACTCCAACCCGACAAGAAAC		 1 7 104–117 0.58 0.61 0.66 GTC5 FJ373286
Crest2T2.56 F: AAATTGCGAAAGGACGATTC
R: TCGATGCTTGAAGTTTATCACG(N)		 2 13 182–218 0.71 0.86 0.4 TGC9 FJ373287
Crest2T4.30 F: CCGGAAGTGAACCGCAAC(H)
R: TGTTGAAACCCACTGTTCTAAACC		 2 14 164–185 0.7 0.74 0.45 TC9 FJ373288
Crest2T4.71 F: TCTCGTTTCGTCCATTCTCC
R: AACCACGCACGATGAGATTC(H)		 3 11 71–97 0.75 0.82 0.75 GTC5 FJ373289

F and R indicate forward and reverse primer. The letter after the primer sequence (F, H, or N) indicates that primer is labelled with blue, green, or yellow fluorescence, respectively, and M indicates which multiplex it is part of. Number of alleles (A), their size range, observed (HO) and expected (HE) heterozygosities, and the P value of the comparison (P) were estimated from a sample of 44 individuals. We list the microsatellite motif we focused on, but other microsatellite types were often present in the sequences in GenBank.

*

This locus amplifies in Culex pipiens. We have found a single allele (186) not found in Culex restuans.