Table 3.

Formulation of the Ham’s F-12K medium without phenol-red and without glutamine, pH 7.4–7.5

Inorganic salts	mg/L	Other compounds	mg/L
NaCl	7,530	Glucose	1,260
KCl	285	Linoleic acid	0
MgCl2–6H2O	106	Hypoxanthine–Na	4
MgSO4–7H2O	393	Phenol-red	0
CaCl2	135	Putresine–HCl	0.3
Na2HPO4–7H2O	218	Sodium pyruvate	220
KH2PO4	59	Thymidine	0.7
NaHCO3	2,500
FeSO4–7H2O	0.8
CuSO4–5H2O	0.002
ZnSO4–7H2O	0.14

Amino acids	mg/L	Vitamins	mg/L
L-Alanine	17.8	L-Ascorbic acid	0
L-Arginine–HCl	421.3	Biotin	0.07
L-Asparagine–H2O	30	D-Calcium pantothenate	0.48
L-Aspartic acid	26.6	Choline chloride	13.96
L-Cysteine–HCl–H2O	70.04	Cyanocobalamin	1.36
L-Cystine	0	Folic acid	1.32
L-Glutamic acid	29.4	Inositol	18
Glycine	15	Nicotinamide	0.04
L-Histidine–HCl–H2O	41.9	Pyridoxine–HCl	0.06
L-Isoleucine	7.9	Riboflavin	0.04
L-Leucine	26.2	Thiamine–HCl	0.21
L-Lysine–HCl	73.1	DL-Thioctic acid	0.21
L-Methionine	8.9
L-Phenylalanine	9.9
L-Proline	69.1
L-Serine	21
L-Threonine	23.8
L-Tryptophan	4.1
L-Tyrosine	10.9
L-Valine	23.4