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. Author manuscript; available in PMC: 2011 Dec 18.
Published in final edited form as: J Neurosci Res. 2009 Apr;87(5):1057–1068. doi: 10.1002/jnr.21937

Figure 2. Cortactin colocalizes with F-actin in Aplysia growth cones.

Figure 2

Cortactin and F-actin show a strong colocalization in the growth cone peripheral domain (P) and transition zone (T) when compared to the central domain (C). A: DIC image of a fixed growth cone. B: F-actin staining of the same growth cone using Alexa 488 phalloidin revealed filopodial bundles (arrowheads), lamellipodial veils at the leading edge (arrows) and T zone ruffles (R). C: Cortactin staining with 4F11. D: Overlay of F-actin and cortactin staining. Inset: zoom of the boxed region showing strong actin/cortactin colocalization in filopodia and the lamellipodia at the leading edge. Bar: 10 μm (5 μm for inset).