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. Author manuscript; available in PMC: 2011 Dec 18.
Published in final edited form as: J Neurosci Res. 2009 Apr;87(5):1057–1068. doi: 10.1002/jnr.21937

Figure 4. Cortactin and Src colocalization in growth cones.

Figure 4

Cultured Aplysia bag cells were fixed, extracted, and double labeled for cortactin together with Src1 (A), Src2 (B), activated Src2 (pSrc2; C), or triple labeled for cortactin together with F-actin and activated Src2 (D). Regions in magenta denote the colocalization of F-actin and cortactin. Note the enriched pSrc2 staining at the tip of filopodia when compared to total Src2 (arrows in C and D). E–F: Triple staining for cortactin, F-actin, and pSrc2 after live cell extraction. Cortactin was strongly removed from actin bundles in the P domain, but remained in the ruffling zone (E). Activated Src2, on the other hand, remained at the tip of filopodia (arrows in F), which were identified by F-actin staining. The pSrc2 staining outside the growth cone (mostly at the top of image F) is due to cell debris accumulated during the plasma membrane removal. Bars: 10 μm.