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. 1980 Sep 11;8(17):3865–3874. doi: 10.1093/nar/8.17.3865

DNA gyrase subunit stoichiometry and the covalent attachment of subunit A to DNA during DNA cleavage.

A Sugino, N P Higgins, N R Cozzarelli
PMCID: PMC324200  PMID: 6255421

Abstract

Escherichia coli DNA gyrase contains a 1:1 ratio of protomers coded by the genes gyrA and gyrB. This along with previous results shows that the enzyme has two copies of each protomer and thus a molecular weight of 400,000. Abortion of the gyrase reaction results in double-strand breakage of the DNA and covalent attachment of both gyrA protomers to the 5'-cut ends. We conclude that the gyrA protomer contains a critical part of the active site for the concerted breakage and reunion reaction of gyrase, the topoisomerase activity of the enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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