Table 1. Link between mutations in antigenic site and RBS residues for HA-escape mutants10. SA-anchoring RBS residues contained within the SIN of antigenic residues whose SIN score is generally higher than corresponding score in wild-type HA as a result of antigenic mutations that increase glycan-binding affinity are highlighted in yellow. Highlighted in cyan are SA-anchoring residues contained within the SIN of antigenic residues whose SIN score is generally lower than corresponding score in wild-type HA as a result of antigenic mutations that decrease glycan-binding affinity. In the case of mutations that do not alter the glycan-binding affinity, most of these residues do not have any SA-anchoring RBS residues in their SIN. The SA-anchoring RBS residues (whose SIN scores are not affected) are in the network of these antigenic mutations are shown in red text.
| Emergent Mutation | SIN Score | RBS Residues in SIN |
|---|---|---|
| Higher Affinity than WT | ||
| L74P | LOW | Q226 |
| L75P | LOW | Y98 |
| E119G | LOW | Y98, T136 |
| P128S | LOW | G134, T136 |
| N129K | LOW | H183, T155, W153 |
| G143R | LOW | Y98, T136 |
| E156G | LOW | Y98, W153, T155, H183 |
| E156K | LOW | Y98, W153, T155, H183 |
| E158V | LOW | W153, T155 |
| L164Q | LOW | W153, H183, Y98, Q226 |
| K165E | LOW | H183, E190 |
| N166K | LOW | H183, L194 |
| Q192R | LOW | W153, T155 |
| N193K | LOW | T136 |
| Q196R | LOW | T155, W153, H183, Y98, Q226 |
| E198G | LOW | H183, L194 |
| R224I | LOW | Y98, W153, H183, Q226 |
| I244T | LOW | H183, L194 |
| Lower Affinity than WT | ||
| I93T | LOW | Y98, T136, W153, H183, Q226 |
| S145G | LOW | Y98 |
| S145N | LOW | Y98, T136 |
| N193S | LOW | W153 |
| Similar Affinity as WT | ||
| V77E | LOW | _ |
| R78G | LOW | _ |
| S79P | LOW | _ |
| E119K | LOW | Y98, T136 |
| N129Y | LOW | H183, T155, W153 |
| S140P | LOW | _ |
| G159D | LOW | _ |
| S160L | LOW | W153, H183 |
| K163T | LOW | W153, H183 |
| S167Y | LOW | _ |
| S167F | LOW | _ |
| V169A | LOW | _ |
| G173E | LOW | _ |
| G173R | LOW | _ |
| Q192L | LOW | W153, T155 |
| G240E | LOW | _ |
| G240R | LOW | _ |