Table 3.

delta CT values for cytokine genes after 24 hours of in vitro culture in the presence or absence of heat killed M. tuberculosis

	Unstimulated					Stimulated					unstimulated vs. stimulated
mRNA	IRIS	IQR	non- IRIS	IQR	p	IRIS	IQR	non- IRIS	IQR	p	IRIS	non- IRIS
IL-1β	6.4	−1.3-7.8	6.7	4.0-8.6	0.202	−2.7	−3.6-1.7	−0.6	−1.9-0.2	<0.001	< 0.001	<0.001
IL-2	11.5	9.0-12.9	12.7	12.2-13.7	0.014	8.3	7.5-9.4	10.0	7.0-12.7	0.129	< 0.001	<0.001
IL-4	15.5	14.9-16.7	16.1	15.3-17.1	0.409	14.4	14.1-15.8	15.4	14.0-16.6	0.382	0.001	0.004
IL-5	20.2	19.3-20.6	18.9	18.0-20.0	0.003	12.1	11.4-14.7	17.0	13.9-20.1	0.001	< 0.001	0.13
IL-6	12.0	4.0-13.8	12.4	9.7-13.9	0.78	1.3	0.3-3.7	4.6	3.1-5.9	<0.001	< 0.001	<0.001
IL-8	3.0	−0.2-3.8	2.6	0.4-5.1	0.48	−3.7	−4.6-2.1	−1.5	−2.8-1.6	0.006	< 0.001	0.002
IL-10	6.9	5.1-8.1	6.9	6.0-7.1	0.74	4.9	3.1-6.4	6.8	5.9-8.5	<0.001	< 0.001	1,0
IL-12p40	16.2	12.1-17.5	16.9	13.9-18.1	0.20	8.1	6.5-9.4	10.0	7.8-13.3	0.005	< 0.001	<0.001
IL-13	17.6	13.5-10.3	17.6	14.8-18.4	0.61	8.8	7.9-10.0	11.0	9.4-13.7	0.002	< 0.001	<0.001
IL-15	9.6	8.6-10.5	10.6	10.1-11.4	0.005	8.4	7.9-8.7	8.6	8.0-9.6	0.14	0.004	<0.001
IL-17A	18.3	16.3-19.5	18.2	17.7-19.4	0.698	12.7	11.5-14.1	13.9	12.8-15.3	0.048	< 0.001	<0.001
IL-18	9.9	9.2-10.4	10.5	9.0-12.6	0.14	9.9	8.9-10.7	9.6	8.6-11.4	0.85	0.45	0.36
IFNγ	9.4	6.0-10.6	9.6	8.6-10.7	0.259	3.3	1.8-5.1	6.8	4.3-8.1	0.002	< 0.001	<0.001
GM-CSF	11.8	8.6-14.3	13.6	10.8-15.0	0.382	2.8	−0.9-3.7	5.0	3.9-6.8	<0.001	< 0.001	<0.001
TGF-β	4.7	4.4-5.4	4.5	4.1-4.9	0.1	4.6	4.3-4.9	4.8	4.5-5.6	0.16	0.18	0.004
TNF	7.3	2.7-7.90	8.2	7.7-8.5	<0.001	1.4	1.1-2.0	3.2	2.4-4.9	<0.001	< 0.001	0.002

p values are uncorrected for multiple comparisons in the table but multiple comparisons we factored in the analysis (see text). Lower values indicate high transcript abundance of the gene of interest