Abstract
The nucleotide sequence of tobacco chloroplast 4.5S ribosomal RNA has been determined to be: OHG-A-A-G-G-U-C-A-C-G-G-C-G-A-G-A-C-G-A-G-C-C-G-U-U-U-A-U-C-A-U-U-A-C-G-A-U-A-G-G-U-G-U-C-A-A-G-U-G-G-A-A-G-U-G-C-A-G-U-G-A-U-G-U-A-U-G-C-(G-A)-C-U-G-A-G-G-C-A-U-C-C-U-A-A-C-A-G-A-C-C-G-G-U-A-G-A-C-U-U-G-A-A-COH. The 4.5S RNA is 103 nucleotides long and its 5'-terminus is not phosphorylated.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bowman C. M., Dyer T. A. 4.5S ribonucleic acid, a novel ribosome component in the chloroplasts of flowering plants. Biochem J. 1979 Dec 1;183(3):605–613. doi: 10.1042/bj1830605. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartley M. R. The synthesis and origin of chloroplast low-molecular-weight ribosomal ribonucleic acid in spinach. Eur J Biochem. 1979 May 15;96(2):311–320. doi: 10.1111/j.1432-1033.1979.tb13042.x. [DOI] [PubMed] [Google Scholar]
- Lockard R. E., Alzner-Deweerd B., Heckman J. E., MacGee J., Tabor M. W., RajBhandary U. L. Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids Res. 1978 Jan;5(1):37–56. doi: 10.1093/nar/5.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peattie D. A. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760–1764. doi: 10.1073/pnas.76.4.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
- Sugiura M., Suzuki M., Ohtsuka E., Nishikawa S., Uemura H., Ikehara M. Purification of T4 RNA ligase by 2', 5'-ADP sepharose chromatography. FEBS Lett. 1979 Jan 1;97(1):73–76. doi: 10.1016/0014-5793(79)80055-1. [DOI] [PubMed] [Google Scholar]
- Sugiura M., Takanami M. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids. II. Comparison of the 5'-terminal nucleotide sequences of ribosomal RNA's from different organisms. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1595–1602. doi: 10.1073/pnas.58.4.1595. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takaiwa F., Sugiura M. Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplasts. Gene. 1980 Jul;10(2):95–103. doi: 10.1016/0378-1119(80)90127-4. [DOI] [PubMed] [Google Scholar]
- Whitfeld P. R., Leaver C. J., Bottomley W., Atchison B. Low-molecular-weight (4.5S) ribonucleic acid in higher-plant chloroplast ribosomes. Biochem J. 1978 Dec 1;175(3):1103–1112. doi: 10.1042/bj1751103. [DOI] [PMC free article] [PubMed] [Google Scholar]