Table 1. Differentially Expressed Candidate Proteins in Hippocampal Tissue Derived From MAM and Sham Rats.
| Swiss Prot ID | Gene name | Peptides quantified | Sequence coverage (%) | Average intensity, MAM±SD | Average intensity, SHAM±SD | Ratio, MAM/SHAM | p-value | RSD QCs (%) | |
|---|---|---|---|---|---|---|---|---|---|
| (a) | |||||||||
| Protein fraction 1 | |||||||||
| Guanine deaminase | Q9WTT6 | GDA | 41 | 76 | 31 338±1839 | 28 491±1490 | 1.09 | 0.0027 | 1 |
| Hippocalcin | P84076 | HPCA | 21 | 86 | 49 947±4522 | 62 606±2655 | 0.80 | 0.0000 | 8 |
| Lactate dehydrogenase A | P04642 | LDHA | 40 | 89 | 85 713±5698 | 94 515±6680 | 0.91 | 0.0058 | 3 |
| Myristoylated alanine-rich protein kinase-C substrate | P30009 | MARCKS | 13 | 60 | 24 461±1511 | 19 924±2094 | 1.21 | 0.0001 | 2 |
| (b) | |||||||||
| Protein fraction 2 | |||||||||
| Amphiphysin | O08838 | AMPH | 22 | 49 | 19 898±1111 | 21 274±1369 | 0.94 | 0.0244 | 2 |
| ARP3 actin-related protein 3 | Q4V7C7 | ACTR3 | 25 | 70 | 24 523±837 | 27 415±1753 | 0.89 | 0.0003 | 8 |
| ATPase, Ca++ transporting, plasma membrane 1 | P11505 | PMCA1 | 69 | 62 | 26 650±990 | 31 272±1463 | 0.85 | 0.0000 | 3 |
| ATPase, Na+/K+ transporting | P06685 | ATP1A1 | 60 | 51 | 81 921±4513 | 77 393±4361 | 1.06 | 0.0342 | 2 |
| Basigin | P26453 | BSG | 8 | 30 | 10 443±1056 | 12 180±830 | 0.86 | 0.0014 | 12 |
| Calcium channel, voltage-dependent, γ-subunit 8 | Q8VHW5 | CACNG8 | 3 | 14 | 20 737±2923 | 24 053±2917 | 0.86 | 0.0188 | 7 |
| Calcium/calmodulin-dependent protein kinase IIα | P11275 | CAMK2A | 31 | 65 | 107 485±10 925 | 12 7287±11 068 | 0.84 | 0.0010 | 6 |
| Calcium/calmodulin-dependent protein kinase IIδ | P15791 | CAMK2D | 17 | 44 | 18 553±1273 | 20 051±1139 | 0.93 | 0.0138 | 6 |
| Calcium/calmodulin-dependent protein kinase IIγ | P11730 | CAMK2G | 17 | 47 | 12 968±670 | 11 998±695 | 1.08 | 0.0058 | 7 |
| Calmodulin | P62161 | CAM | 17 | 89 | 97 711±12 227 | 10 9183±6598 | 0.89 | 0.0257 | 9 |
| Capping protein (actin filament) muscle Z-line, α2 | Q3T1K5 | CAPZA2 | 8 | 42 | 16 601±837 | 18 153±1505 | 0.91 | 0.0124 | 7 |
| Dipeptidyl-peptidase 6 | P46101 | DPP6 | 13 | 26 | 16 133±770 | 17 054±722 | 0.95 | 0.0118 | 7 |
| Dynamin 1 | P21575 | DNM1 | 73 | 81 | 37 638±1047 | 39 025±1555 | 0.96 | 0.0313 | 2 |
| FK506 binding protein 1A, 12 kDa | Q62658 | FKBP1A | 7 | 78 | 30 458±3165 | 36 348±2870 | 0.84 | 0.0009 | 7 |
| Glial high-affinity glutamate transporter member 3 | P24942 | SLC1A3 | 21 | 46 | 61 487±3811 | 57 770±3472 | 1.06 | 0.0335 | 6 |
| Glutamate receptor, ionotropic, and AMPA-1 | P19490 | AMPA1 | 27 | 42 | 14 177±870 | 15w938±659 | 0.89 | 0.0001 | 7 |
| Glutamate receptor, ionotropic, and AMPA 2 | P19491 | AMPA2 | 52 | 62 | 13 719±588 | 14 760±888 | 0.93 | 0.0064 | 6 |
| Glutaminase | P13264 | GLS | 46 | 70 | 22 286±1140 | 24 418±1362 | 0.91 | 0.0013 | 5 |
| Hemoglobin, β | P02091 | HBB | 15 | 83 | 87 376±20 210 | 69 534±12 326 | 1.26 | 0.0217 | 10 |
| Hippocalcin | P84076 | HPCA | 15 | 70 | 32 618±2910 | 38 319±2639 | 0.85 | 0.0003 | 9 |
| Hydroxyprostaglandin dehydrogenase 15-(NAD) | O08699 | HPGD | 5 | 32 | 14214±853 | 15 848±1917 | 0.90 | 0.0354 | 5 |
| Limbic system-associated membrane protein | Q62813 | LSAMP | 4 | 9 | 11 014±1105 | 12 013±659 | 0.92 | 0.0231 | 12 |
| Myosin, heavy chain 4 | Q29RW1 | MYH4 | 27 | 20 | 16 105±510 | 16 803±555 | 0.96 | 0.0093 | 6 |
| Myristoylated alanine-rich protein kinase-C substrate | P30009 | MARCKS | 16 | 63 | 18 180±954 | 14 564±3383 | 1.25 | 0.0218 | 9 |
| Neural cell-adhesion molecule 1 | P13596 | NCAM1 | 46 | 66 | 33 399±1662 | 30 806±1661 | 1.08 | 0.0029 | 5 |
| Peroxiredoxin 6 | O35244 | PRDX6 | 20 | 74 | 22 516±1223 | 20 572±2199 | 1.09 | 0.0294 | 9 |
| Protein phosphatase 2, catalytic subunit, αisoform | P63331 | PPP2CA | 3 | 21 | 4523±815 | 5932±1049 | 0.76 | 0.0033 | 29 |
| Protein phosphatase 3, catalytic subunit, αisoform | P63329 | PPP3CA | 31 | 66 | 37 842±1688 | 41 973±3455 | 0.90 | 0.0042 | 5 |
| RAB14 | P61107 | RAB14 | 23 | 74 | 19 017±1391 | 20 161±707 | 0.94 | 0.0382 | 9 |
| RAB3C | P62824 | RAB3C | 12 | 49 | 40 694±3487 | 36 178±3076 | 1.12 | 0.0076 | 6 |
| RAB8B | P70550 | RAB8B | 7 | 51 | 11 284±837 | 10 442±851 | 1.08 | 0.0393 | 11 |
| Solute carrier family 4, sodium bicarbonate cotransporter, member 4 | Q9JI66 | SLC4A4 | 18 | 29 | 11 815±832 | 11 013±650 | 1.07 | 0.0286 | 4 |
| Synaptic vesicle glycoprotein 2B | Q63564 | SV2B | 3 | 8 | 11 568±1315 | 13 008±1322 | 0.89 | 0.0261 | 17 |
| Synaptojanin 1 | Q62910 | SYNJ1 | 33 | 27 | 26 469±1194 | 29 115±1604 | 0.91 | 0.0006 | 4 |
| Tubulin, α8 | Q6AY56 | TUBA8 | 12 | 36 | 14 945±2221 | 17 439±1984 | 0.86 | 0.0268 | 10 |
| Tubulin, β2B | Q3KRE8 | TUBB2B | 4 | 16 | 22 304±1794 | 20 001±2580 | 1.12 | 0.0331 | 5 |
The proteins in (a) fraction 1 (10 MAM and 10 sham rats) and (b) fraction 2 (10 MAM and 10 sham rats) indicating averaged protein intensities (rounded to integers) and the standard deviation (SD), MAM/sham protein abundance ratios, p-values and relative standard deviation (RSD) for the measurement of each protein (determined by analysis of five quality controls that have been prepared and analyzed with the MAM and sham samples).