Table 2. Primer Sequences Used in this Study.
Genes | 5′ primer | 3′ primer |
---|---|---|
mRNA expression primers | ||
GADD45a | AGAGCAGAAGACCGAAAGGATGGA | GCAGGATGTTGATGTCGTTCTCGC |
GADD45b | ATTGCAACATGACGCTGGAAGAGC | GGATGAGCGTGAAGTGGATT |
GADD45g | GACACAGTTCCGGAAAGCAC | TCAAGACTTTGGCTGACTCG |
MBD4 | AAAACGTGGCTCTGAAATGG | TCTGTGTTCGTGGGATGGTA |
TDG | AACATGGTGGAAAGGACCAC | ACACTGCTATTCGTGGCTGA |
XPG | CTTTGTTTCATCGGCTCTGC | CGTTTTCCTGGAGTCACTGG |
BDNF IXabcd | AACCTTGACCCTGCAGAATG | TGGTCATCACTCTTCTCACCTG |
NSE | TGTCTCTGGCCGTGTGTAAG | GCATGAGAGCCACCATTGAT |
GAPDH | CGAGATCCCTCCAAAATCAA | TTCACACCCATGACGAACAT |
ACTB | TCCCTGGAGAAGAGCTACGA | TGAAGGTAGTTTCGTGGATGC |
TFRC | AAAATCCGGTGTAGGCACAG | CACCAACCGATCCAAAGTCT |
Chromatin immunoprecipitation primers | ||
BDNFIXabcd −60 to +50 | CCTCTGGCAAACAGGAAGAG | CGCGCTCTGAGTTTATCCTA |
BDNFIXabcd +1185 to +1305 | AAACATCCGAGGACAAGGTG | TTCGAAAGTGTCAGCCAATG |
Genes |
Probe |
|
In situ mRNA probes | ||
BDNFIXabcd +514 to +537 | AGCAGTTGGGGAACTATAAGGAG | |
BDNFIXabcd +761 to +784 | AATGATCTCTGCTCATGCTGTC | |
BDNFIXabcd +714 to +740 | GTTCTCCTTCTTCCCACTTTAGC |