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In vitro human RPE GAPDH and nitrotyrosine. Cultured human RPE cells (second passage) were exposed to control medium (A, C) or medium containing 200 µM hydrogen peroxide (B, D) for 24 hours. Immunocytochemistry for GAPDH (A, B) and nitrotyrosine (C, D) was performed. Bar = 10 µm.