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. Author manuscript; available in PMC: 2012 Nov 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Oct 12;415(1):135–140. doi: 10.1016/j.bbrc.2011.10.032

Figure 2. Exogenous erlins form higher-order complexes.

Figure 2

Hela cells were transfected with vectors encoding epitope-tagged erlins, or empty vector, and cell lysates were prepared. In A, lysates were subjected to either native-PAGE or SDS-PAGE and were probed as indicated, with the migration positions of molecular mass markers indicated (masses in kDa). In B, lysates were incubated with anti-E2 or rabbit polyclonal anti-HA [10] and immunoprecipitates were subjected to SDS-PAGE and were probed as indicated. Note that mouse monoclonal anti-E1 is human specific [9,12] and recognizes endogenous E1, but not exogenous E1HA. E1, E2, E1HA and E2HA migrated, respectively, at 41,43, 42 and 44 kDa.