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. Author manuscript; available in PMC: 2012 Nov 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Oct 12;415(1):135–140. doi: 10.1016/j.bbrc.2011.10.032

Figure 3. Effects of mutations to the E2 assembly domain on higher-order complex formation.

Figure 3

Hela cells were transfected with vectors encoding E2HA and its mutants, (A) either without E1HA or with E1HA, or (B) either without or with E1flag. Cell lysates were prepared and were subjected to native-PAGE and were probed with anti-HA or anti-flag as indicated. The migration positions of molecular mass markers are indicated (masses in kDa).