Figure 4. Cross-linking of erlins.
A and B. Hela cells were transfected with the vectors indicated, were exposed to 1mM DSS for 1min, and cell lysates were subjected to SDS-PAGE and were probed with anti-HA. C. E1/E2 complex immunopurified from αT3 cells (lanes 2–7) and control material lacking E1/E2 complex (lanes 1 and 8) were exposed to DSS for 2 min as indicated and subjected to SDS-PAGE and probed with anti-E2 and rabbit polyclonal anti-E1 [6]. The migration positions of molecular mass markers are indicated (masses in kDa) as are the positions of unmodified and cross-linked erlins and IgG heavy chain.