Table 2.
Select proteins identified via proteomics with International Protein Index (IPI) accession numbers, function, and mass spectrometry Protein Prophet (PP) scores
| PP score† |
||||||
|---|---|---|---|---|---|---|
| Accession number | Protein | Function* | IgG | P-sel-Ig | No stimulus | LPS |
| IPI00021439 | β-Actin | Cytoskeletal structure | 1 | 1 | 1 | 1 |
| IPI00013808 | α-Actinin-4 | Actin binding | 0.99 | 1 | 1 | 1 |
| IPI00298994 | Talin | Actin, integrin binding | 1 | 1 | 1 | 1 |
| IPI00005161 | p34arc | Arp 2/3 subunit; actin filament branching |
ND | 1 | ND | ND |
| IPI00000477 | ND | 0 | ND | 1 | ||
| IPI00554811 | p20arc | Arp 2/3 subunit; actin filament branching |
ND | ND | ND | 1 |
| IPI00642191 | 1 | 1 | 1 | 0 | ||
| IPI00657756 | LAIR-1 | Inhibitory immune regulation | ND | 0.99 | ND | ND |
| IPI00000190 | CD81 | Proliferation regulation | 1 | 1 | ND | 1 |
| IPI00155168 | CD45 | Tyrosine phosphatase | 1 | 1 | 1 | 1 |
| IPI00291792 | β2-Integrin | Cell adhesion/signaling | 1 | 1 | 1 | 1 |
| IPI00644788 | αL-Integrin | Cell adhesion/signaling | 1 | 1 | 1 | 1 |
| IPI00217563 | β1-Integrin | Fibronectin receptor | 0 | 0.95 | ND | 0 |
| IPI00645194 | 1 | ND | ND | 1 | ||
| IPI00306604 | α5-Integrin | Fibronectin receptor | 0.9 | 1 | 0.97 | 1 |
| IPI00412656 | β7-Integrin | Cell adhesion | ND | ND | 0 | 1 |
| IPI00299412 | CD97 | Inflammatory response regulator |
ND | 0.99 | ND | ND |
| IPI00253036 | CD99 | Cell adhesion | ND | 0.97 | ND | ND |
| IPI00021854 | ApoAII | Lipoprotein metabolism | ND | 1 | ND | ND |
| IPI00414264 | Sorcin | Ca2+ binding | ND | 0.96 | ND | 1 |
| IPI00002460 | Annexin a7 | Ca2+ gate regulation | ND | ND | 1 | 1 |
| IPI00218733 | Superoxide dismutase | Oxidative stress response | ND | 1 | ND | 1 |
| IPI00216231 | Snap23b | t-SNARE activity | ND | 1 | ND | 0.95 |
| IPI00465315 | Cytochrome c | Electron transport | ND | 0.99 | ND | 1 |
| IPI00514407 | HMGCL | Metabolic activity | ND | 0.99 | ND | 0.99 |
| IPI00294779 | VDAC3 | Mitochondrial voltage gate regulation |
ND | 0.86 | 1 | 1 |
| IPI00420014 | 200-kDa helicase | Nuclear splicing | ND | ND | ND | 0.99 |
ApoAII, apolipoprotein AII; Arp, actin-related protein; Snap23b, synaptosomal-associated protein 23; HMGCL, 3-hydroxymethyl-3-methyl-glutaryl-coenzyme A lyase; LPS, lipopolysaccharide; P-sel-Ig, P-selectin-Ig chimera; VDAC3, voltage-dependent anion-selective channel protein 3.
*
Functions based on Gene Ontology analysis.
†
PP scores range from ND (not detected) and 0 to 1 [19].
Proteins with PP scores ≥ 0.9 were considered to be present.