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. 2011 Dec 14;441(Pt 1):317–323. doi: 10.1042/BJ20111617

Figure 4. Oligomerization of TPCs.

Figure 4

(A) Top panels: Western blot analysis using an anti-GFP antibody of immunoprecipitates prepared using an anti-Myc antibody from cells co-expressing GFP- and Myc-tagged domain I (DI), loop (L) or domain II (DII) of the indicated isoform. Western blot analysis using an anti-GFP or anti-Myc antibody of the detergent extracts prior to immunoprecipitation are shown in the middle and bottom panels respectively. (B) Western blot analysis using an anti-GFP antibody of immunoprecipitates prepared using an anti-Myc antibody from cells co-expressing the indicated GFP- and Myc-tagged TM regions of TPC1. Data are representative of 2–11 experiments. The molecular mass in kDa is indicated on the left-hand side. IB, immunoblot; IP, immunoprecipitation.