Table 1. Summary of domain cross-linking experiments.
The size of the monomeric and dimeric sized bands and the ratio (dimer/monomer) is shown for each domain construct analysed. Results are means±S.E.M. from n experiments.
| Isoform | Domain | Construct | Monomeric size (kDa) | Dimeric size (kDa) | Ratio | n |
|---|---|---|---|---|---|---|
| TPC1 | Domain I | TPC11–347–GFP | 66±1 | 138±4 | 2.1±0.07 | 7 |
| TPC1 | Domain II | TPC1427–816–GFP | 70±1 | 156±5 | 2.2±0.06 | 4 |
| TPC2 | Domain I | TPC21–339–GFP | 54±2 | 125±2 | 2.3±0.07 | 3 |
| TPC2 | Domain II | TPC2422–752–GFP | 57±2 | 126±2 | 2.2±0.05 | 3 |