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. 2011 Dec 14;441(Pt 1):481–485. doi: 10.1042/BJ20111258

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Circular representation of pRW901 showing the most important features. The plasmid carries five DNA sites for LacI (LacO) upstream of a polylinker flanked by EcoRI and HindIII restriction sites. (B) DNA base sequence of the constitutively active KAB-CGTG promoter [11] inserted between the EcoRI and HindIII sites to give pRW904. The promoter −10 and −35 hexamer elements are in bold and underlined.