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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Immunol. 2011 Nov 4;187(11):5805–5812. doi: 10.4049/jimmunol.1100338

Figure 2.

Figure 2

Regulation of C5aR expression on NK and NKT cells by infection. (A+B) NKT and NK cells express higher levels of C5aR mRNA than of C5L2 mRNA. Total RNA of TCRβ+ CD1d-tetramer+ NKT (A) and Nkp46+ NK1.1+ NK (B) cells (purity >98%) from naive mice was analyzed for their C5aR and C5L2 expression profile by RT-PCR as well as one and four hrs after E. coli-infection (one out of two experiments containing three individual mice each). Note the 10–50 time higher C5aR copy numbers in uninfected and infected mice despite the lack of regulation of C5aR expression. Statistical significance was calculated using ANOVA. The experiment was repeated once with similar results. (C-D) C5aR protein is expressed on the cell surface 24h after infection. Analysis of cell surface C5aR expression on NKT (C) and NK cells (D) by flow cytometry. Shown is a typical expression profile from one uninfected and one infected C5aR−/− and one wt B6 mouse out of 4. (E) Frequency of C5aR+ NKT and NK cells in uninfected and E. coli–infected mice (n=4). The experiment was repeated once with similar results.