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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Immunol. 2011 Nov 4;187(11):5805–5812. doi: 10.4049/jimmunol.1100338

Figure 6.

Figure 6

Crosstalk of C5aR and defined TLRs on DCs and NKT as well as DCs and NK cells drives synergistic TNF-α and IFN-γ production. (A+B) C5aR and TLR-mediated cross-talk between DCs and NKT (A, left panel) cells promotes early TNF-α production whereas cross-talk between DCs and NK cells (B) drives IFN-γ production in early E. coli-mediated sepsis. Sorted NKT cells (purity >98%), NK cells (purity >95%) as well as BM-derived DCs from infected B6 or C5aR−/− were exposed to the indicated TLR ligands. Cytokine release was determined 48 h later by ELISA. Monocultures of DCs (A, middle panel) and of NKT cells (A, right panel) produced TNF-α. In contrast, neither monocultures of BM-derived DCs nor of purified NK cells produced IFN-γ (not shown). Statistically significant differences were determined using ANOVA. The experiment was repeated once with similar results.