Figure 1.

Effect of doubling dilutions of gemcitabine (A), afatinib (B) or erlotinib (C) on the growth of human pancreatic cancer cells. Tumour cells were grown in growth medium (2% FBS) with the inhibitors or medium alone until control cells (only medium) were confluent. Cancer cell proliferation was calculated as a percentage of control cell growth, as described in the Materials and Methods. Each point represents the mean±s.d. (D) The effect of gemcitabine at 100 nM, ICR62 at 200 nM and erlotinib or afatinib at 1.5 μM in pancreatic cancer cell lines (as percentage of control growth) is shown (columns, mean of triplicate values; bars, ±s.d.).