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. 2011 Oct 11;105(10):1582–1592. doi: 10.1038/bjc.2011.403

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Copyright © 2011 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

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Representative sections of early invasive oral carcinoma (IOC) taken from patient biopsies (A–E) and a tissue-engineered invasive carcinoma (TEIOC) (F–J). TEIOC were generated by culturing OSCC (Cal27) and normal oral fibroblasts on DED at an air/liquid interface for 21 days. Sections have been stained with H&E (A and F) and immunohistochemically stained to show the pattern of expression of Ki67 (B and G), AE1/3 (C and H), collagen type IV (Col IV; D and I) and integrin αvβ6 (E and J) and counterstained with haematoxylin. Scale bar indicates 200 μm.