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. 2011 Oct 20;105(10):1563–1573. doi: 10.1038/bjc.2011.426

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Copyright © 2011 Cancer Research UK

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Knockdown of c-Myc levels augments the activity of SGI-1776 and sunitinib. (A) The combination of SGI-1776 and sunitinib stimulates a further reduction in phosphorylated and total c-Myc levels. Cells were treated with 5 μM SGI-1776, 5 μM sunitinib, or the combination for 48 h. Phospho- and total c-Myc levels were detected by immunoblotting. Phospho- and total c-Myc levels were quantified by densitometry using ImageJ software. (B) Silencing c-Myc expression using siRNA. Cells were transfected with non-target control or c-Myc siRNA and c-Myc expression levels were determined by immunoblotting at 48 h. Band intensity was quantified using ImageJ software. (C) Knockdown of c-Myc enhances SGI-1776 and sunitinib-mediated apoptosis. 786-O cells were transfected with non-target control or c-Myc siRNA and then treated with 5 μM SGI-1776, 5 μM sunitinib, or the combination for 48 h. Apoptosis was determined by PI-FACS analysis. Mean±s.d., n=3. ^*Indicates a significant difference compared with the non-target transfected cells treated under the same conditions. P<0.05.