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. 2011 Oct 20;105(10):1615–1624. doi: 10.1038/bjc.2011.431

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Copyright © 2011 Cancer Research UK

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Immunoblotting analysis of endostatin in the lung tissues of WT and MMP-13 KO mice. (A) Representative data of immunoblotting of endostatin in the lung tissues of WT and MMP-13 KO mice. Mice were received intravenous injection of B16BL6 cells and lung tissues obtained on days 0 (Cont), 1, 3 and 7 after the injection were subjected to immunoblotting for endostatin as described in Materials and Methods. β-Actin, a loading control. (B) Time-course changes of endostatin/β-actin ratio on days 0 (Cont), 1, 3 and 7 (n=5 per group). Densitometric analysis was carried out using Image J software. Bars, mean±s.d. ^*P<0.05.