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. 2011 Oct 20;105(11):1750–1758. doi: 10.1038/bjc.2011.434

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Copyright © 2011 Cancer Research UK

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Differential display of OCUM-2MD3 cells treated with LA and vehicle. (A) Differential display of the amplified cDNA products from LA-treated cells and vehicle-treated cells. Arrows indicate base pair. (B) Homology results of the differential display band by NCBI BLAST. PAI-1 mRNA-binding protein shows highest score. (C) PAI–RBP mRNA expression 6 h after oligo RNAi and control RNAi treatment as measured by real-time RT–PCR. PAI–RBP mRNA expression was suppressed to 48% by oligo RNAi. (D) PAI-1 mRNA expression 24 h after PAI–RBP, and control oligo RNAi treatment as measured by real-time RT–PCR. PAI-1 mRNA expression was suppressed to 70% by PAI–RBP knockdown. Asterisk indicates P<0.05. Values shown are mean±s.d. (E) PAI-1 protein expression 48 h after PAI–RBP and control oligo RNAi treatment. PAI-1 protein expression was suppressed by PAI-1 mRNA-binding protein knockdown.