Table 1. Primers for detecting and quantifying human and turkey cells using PCR.
Gene | Name | 5′ → 3′ sequence |
---|---|---|
Human alpha satellite | Cr17_1aa | GGGATAATTTCAGCTGACTAAACAG |
Cr17_1bb | TTCCGTTTAGTTAGGTGCAGTTATC | |
Cr17_4bc | AAACGTCCACTTGCAGATTCTAG | |
Avian GAPDH | Forward | GAGGAAAGGTCGCCTGGTGGATCG |
Reverse | GGTGAGGACAAGCAGTGAGGAACG |
Abbreviation: PCR=polymerase chain reaction.
Forward primer.
Reverse primer for conventional PCR.
Reverse primer for quantitative PCR.