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. 2011 Nov 1;105(11):1759–1767. doi: 10.1038/bjc.2011.449

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Copyright © 2011 Cancer Research UK

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Effect of CM from cultures enriched for CSCs on crc cell proliferation and survival. (A) Effect of CM from side-population-based CSCs on growth of HT29 parental cells. Conditioned media were obtained from parental HT29 (control CM) or cells enriched for the CSC phenotype by the side-population assay. These media were used to treat HT29 cells for 48 h, and then MTT assays were performed on triplicate cultures. (B) Effect of conditioned media from Aldefluor-based enrichment for CSCs on growth of HT29 parental cells. Conditioned media were obtained from HT29 sorted by the Aldefluor assay for Aldefluor-negative (ALDH neg CM) and Aldefluor-positive (ALDH pos CM) cells. These media were used to treat HT29 cells for 48 h, and then MTT assays were performed on triplicate cultures. ^*P<0.05.