Figure 3.

Recovery of mitochondriyal mass and function by blocking autophagy. (A–C) Rat2 cells were exposed to bafilomycin A (0.5 and 1 nM) or 3MA (1 to 3 mM) 1 h prior to K-Ras^V12 infection and further incubated for 3 d. (A) Mitochondrial (left part) and lysosomal (right part) mass were quantitated by flow cytometric analysis after co-staining cells with MitoR and LysoG. (B) western blot analyses for the recovery of respiratory proteins by pretreatment of bafilomycin A (left part) and 3MA (right part). (C) Maximum cellular oxygen consumption rates recovered by pretreatment of bafilomycin A (1 nM) and 3MA (3 mM). (D) Cellular oxygen consumption rates was estimated after Rat2 cells were transfected with si-ATG5, si-VATPaseE and si-Beclin 1 15 h prior to K-Ras^V12 infection and further incubated for 3 d. **p < 0.01 vs. MFG control and ##p < 0.01 vs. K-Ras-infected cells by one-way ANOVA.