FIG. 5.

Detection of bacteria by DNA hybridization in sample 200. Microarray image showed positive fluorescent spots (top figure) corresponding to universal eubacterial probes (UNI1392, EU338, Y2R), high-GC-content Gram-positive bacteria (Actinomycetes group, probe HGC236), the low-GC-content Gram-positives Firmicutes including the Lactobacillus subgroup (LGC354a), the Bacillus subgroup (LGC354b), and the Streptococcus subgroup (LGC354c); Deltaproteobacteria (DELTA495a); and Archaea (1Af). This last probe corresponds to a highly degenerated oligonucleotide considered specific for Archaea. Because the fluorescent probe was amplified with primers for Eubacteria, we did not expect positive results with 1Af unless it came from similar sequences from unknown bacteria. (Bottom figure) Plot showing the quantification of fluorescent signals from nonsaturated images. Color images available online at www.liebertonline.com/ast