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. 2011 Jul 19;19(12):2133–2143. doi: 10.1038/mt.2011.131

Figure 5.

Figure 5

Eradication of HER2-expressing tumor cells by HER2-CTLs. On day 7 after the 4th LCL-stimulation, 1 × 106 HER2-CTLs were cocultured with 1 × 106 HER2+ U373 cells. CTLs and U373 cells were plated alone in TCM without cytokines in each well of a 24-well plate as controls. Five days later, cultures were stained with anti-CD3-FITC antibody and anti-Her2/neu-PE antibody and immediately analyzed by flow cytometry. CTLs, cytotoxic T lymphocytes; HER2, human epidermal growth factor receptor 2; LCLs, B lymphoblastoid cell lines.