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. 2011 Sep 13;19(12):2258–2268. doi: 10.1038/mt.2011.167

Figure 1.

Figure 1

Generation of multi-TAA-specific CTL. Peripheral blood mononuclear cells were stimulated with autologous dendritic cells loaded with pepmixes spanning SSX2, Survivin, and MAGE-A4 in the presence of IL-7, IL-12, IL-15 + IL-6, + IL-27, + IL-6/ IL-27, + IL-18 or + IL-6/ IL-18. (a) The specificity of multi-TAA CTL lines generated from five donors as measured by IFN-γ release (IFN-γ ELIspot) in response to stimulation with the pepmix antigens SSX2 (black bars), Survivin (gray bars), and MAGE-A4 (dark gray bars) is shown. Results are expressed as spot-forming cells (SFC)/2 × 105, input cells ± SEM. Control was IFN-γ release in response to stimulation with irrelevant pepmix (white bars). (b) Phenotype of the multi-TAA-specific CTLs. (c) CTL proliferation was measured by cell counting, using trypan blue exclusion at days 0, 9 and 16 after stimulation (n = 4 donors). (d) TAA specificity was designated positive if SFC ≥2x of control activity. CTL, cytotoxic T lymphocyte; IFN-γ, interferon-γ IL, interleukin; TAA, tumor-associated antigen.