Table 3.
Associations between SNPs in the CD46, SLAM and CD209 genes and humoral immune response to measles vaccine in the combined, Caucasian and African-American study subjects
| Gene | SNP ID | Location/function | Geno-typea | n | Median antibody titer mIU/ml (IQR)b | p valuec |
|---|---|---|---|---|---|---|
| Combined cohort of subjects (n = 745) | ||||||
| CD46 | rs2724384* | intron | AA | 450 | 978 (440; 1,957) | 0.0007 |
| AG | 264 | 736 (402; 1,630) | ||||
| GG | 30 | 522 (274; 706) | ||||
| CD46 | rs11118516 | intron | GG | 647 | 807 (405; 1,723) | 0.0161 |
| GA | 92 | 1,069 (500; 2,296) | ||||
| AA | 1 | 4,505 (4,505; 4,505) | ||||
| CD209 | rs8112310 | flanking 3′ UTR | TT | 505 | 803 (401; 1,650) | 0.0333 |
| TA | 224 | 954 (433; 2,087) | ||||
| flanking 5′ UTR |
AA AA | 0 |
– |
– |
||
| CD209 | rs735239 | 360 | 962 (428; 1,929) | 0.0395 | ||
| AG | 314 | 759 (400; 1,638) | ||||
| GG | 67 | 800 (460; 1,635) | ||||
| CD209 | rs12611071 | flanking 5′ UTR | CC | 509 | 800 (400; 1,638) | 0.0444 |
| CA | 199 | 1,059 (477; 2,209) | ||||
| AA | 35 | 712 (279; 2,272) | ||||
| Caucasian subgroup (n = 598) | ||||||
| CD46 | rs2724384* | intron | AA | 344 | 996 (464; 1,943) | 0.0008 |
| AG | 227 | 782 (401; 1,638) | ||||
| GG | 27 | 533 (274; 706) | ||||
| CD209 | rs12611071 | 3′ intergenic | CC | 454 | 801 (401; 1,683) | 0.0303 |
| CA | 131 | 1,185 (523; 2,119) | ||||
| AA | 13 | 645 (329; 2,849) | ||||
| CD209 | rs8112310 | flanking 3′ UTR | TT | 450 | 803 (402; 1,685) | 0.0497 |
| TA | 139 | 1,059 (451; 2,008) | ||||
| AA | 0 | – | ||||
| African-American subgroup (n = 89) | ||||||
| CD46 | rs11118516 | intron | GG | 76 | 619 (268; 1,947) | 0.0028 |
| GA | 13 | 1,690 (848; 4,120) | ||||
| CD209 | rs4804805 | flanking 5′ UTR | AA AA | 0 64 | 1,059 (446; 2,546) | 0.0042 |
| AG | 21 | 279 (193; 531) | ||||
| GG | 4 | 1,025 (365; 1,735) | ||||
| CD209 | rs7252229 | intron | GG | 45 | 510 (251; 928) | 0.0394 |
| GC | 36 | 1,481 (532; 2,786) | ||||
| CC | 8 | 1,263 (349; 2,519) | ||||
| CD209 | rs735239 | flanking 5′ UTR | AA | 68 | 914 (334; 2,380) | |
| AG | 20 | 533 (199; 1,382) | 0.0419 | |||
| GG | 1 | 327 (327; 327) | ||||
A = Adeninę; C = cytosine; G = guanine; T = thymine; IQR = interquartile range.
A total of 55 SNPs were examined; only those found to be statistically significant (p < 0.05) were included in the table. SNP rs8112310 in the combined cohort and in the Caucasian subgroup displayed violations of H WE (p = 8.5488E−07 and p = 0.0012, respectively).
Values are presented as homozygous major allele/heterozygous/homozygous minor allele.
Values are median levels (IQR) in mIU/ml as measured by PRMN assay.
Test for trend p value from the analysis of covari-ance adjusting for age, gender, race and age of immunization. The p values are adjusted for age, gender, race (when necessary), age at the first and second MMR and cohort status (cohort 1 vs. cohort 2) using analysis of covariance methodology.
Previously reported SNP associated with measles antibodies [24].